Wambina Nature Reserve protects a variety of habitats from ridges of open forest to deep rainforest gullies. These habitats in turn support a diverse range of flora and fauna including species of ecological significance on a local, regional and state-wide scale.
Twelve animal species found in the reserve are listed as Vulnerable (Schedule 2) under the Threatened Species Conservation Act 1995, including a nursery and mating colony of the Grey-headed Flying-fox. In addition, twelve plant species identified within the reserve are of special conservation significance.