Lane Cove River is situated within the Sydney Metropolitan region of New South Wales. This estuary is classed as a drowned valley and flows into Sydney Harbour from the north-western part of Greater Sydney.
Lane Cove River winds through a bushland valley that plays a huge ecological role in a largely urbanised area. Significant areas of mangroves flank the tidal estuary up to a weir located near Fullers Road Bridge, above which runoff enters the main channel from urban streams and drains.